Ying Zhang
Professor
Address: Bldg.8 Rm 1424
Medical Research Institute
Wuhan University
Wuhan, Hubei Province, P.R.China 430071
Email: Ying.Zhang84@whu.edu.cn
EDUCATION AND APPOINTMENTS
2018- Professor, Medical Research Institute, Wuhan University, China
2015-2018, Senior Scientist, Group Lead of HDR in HSC, CRISPR Therapeutics Inc.
2012-2015, Postdoctoral fellow, Dept. of Genetics, Harvard Medical School
2006-2012, Ph.D. in Cell, Stem Cell, and Developmental Biology, University of Colorado Anschutz Medical Campus
2002-2006, B.S. in Biology, Nankai University, Tianjin, China
OVERVIEW
Our ultimate goal is to translate CRISPR technologies into cell therapy by either directly restoring pathogenic mutations within cells or by manipulating immune cell to enhance its anti-tumor ability for cancer treatment. We aim to build and engineer a complete suite of toolbox for cellular genome manipulation that can be safely delivered into primary cells for precision medicine.
Area of interest:
1. CRISPR/Cas9 toolbox discovery and engineering;
2. Non-viral delivery of macromolecules for cell therapy;
3. CRISPR/Cas9 mediated cell therapy including immunotherapies and genetic disorders.
SELECTED PUBLICATIONS (*Corresponding author)
1. W Han#, HY Qiu#, S Sun#, ZC Fu#, GQ Wang#, X Qian, L Wang, X Zhai, J Wei, Wang Y, Guo YL, Cao GH, Ji RJ, Zhang YZ, Ma H, Wang H, Zhao M, Wu J, Bi LL, Chen QB, Li Z, Yu L, Mou X, Yin H, Yang L*, Chen J*, Yang B*, Zhang Y*. Base editing of the HBG promoter induces potent fetal hemoglobin expression with no detectable offtarget mutations in human HSCs. Cell Stem Cell, 30,1-16, Dec 7th, 2023
2. J An #, CP Zhang#, HY Qiu#, HX Zhang#, QB Chen, YM Zhang, XL Lei, CX Zhang, H Yin*, Y Zhang*. Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS-STING pathway. Nature Biomedical Engineering, July 27, 2023
3. CP Zhang, HY Qiu, CX Zhang, YM Zhang, YZ Zhang, H Yin, KQ Zhang*, Y Zhang*. Efficient non-viral delivery of macromolecules in human primary hematopoietic stem cells and lymphocytes. Journal of Molecular and Cell Biology, 2023 Aug 3;15(3)
4. HX Zhang#, CX Zhang#, SH Lu, XH Tong, K Zhang, H Yin*, Y Zhang*. Cas12a-based one-pot SNP detection with high accuracy. Cell Insight, (2023): 100080.doi.org/10.1016/j.cellin.2023.100080
5. HY Qiu, RJ Ji, Y Zhang*. Current advances of CRISPR-Cas technology in cell therapy. Cell Insight, (2022): 100067doi.org/10.1016/j.cellin.2022.100067
6. YJ Shi#, M Duan#, JM Ding#, FQ Wang, LL Bi, CX Zhang, YZ Zhang, JY Duan, AH Huang, XL Lei, H Yin, Y Zhang*. DNA topology regulates PAM-Cas9 interaction and DNA unwinding to enable near PAMless cleavage by thermophilic Cas9. Molecular Cell, Nov 3;82(21):4160-4175, 2022
7. SH Lu#, XH Tong#, Y Han#, K Zhang, YZ Zhang, QB Chen, JY Duan, XL Lei, MH Huang, Y Qiu, DY Zhang, X Zhou, Y Zhang* and Hao Yin*. Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a. Nature Biomedical Engineering, 2022 Mar;6(3):286-297, 2022
