Ke Lan, M.D., Ph.D.
Professor and Deputy Director, Medical Research Institute
Tel: 0086-87688345
Email: klan@whu.edu.cn
Research Areas:
Mechanisms of Viral latency control and oncogenesis
Selected Publications:
1. Liu XJ, Feng JP, Zhang QH, Guo D, Zhang L, Suo T, Hu WJ, Guo M, Wang X, Huang ZX, Xiong Y, Chen GZ, Chen Y*, Lan K*. Analytical Comparisons of SARS-COV-2 Detection by qRT-PCR and ddPCR With Multiple Primer/Probe Sets. Emerg Microbes Infect. 2020 May 24;1-12. doi: 10.1080/22221751.2020.1772679.Online ahead of print.
2. Suo T, Liu XJ, Feng JP, Guo M, Hu WJ, Guo D, HafizUllah , Yang Y, Zhang QH, Wang X, Sajid M, Huang ZX, Deng LP, Chen TL, Liu F, Xu K, Liu Y, Zhang Q, Liu YL, Xiong Y, Chen GZ, Lan K*, Chen Y*. ddPCR:A More Accurate Tool for SARS-CoV-2 Detection in Low Viral Load Specimens. Emerg Microbes Infect. 2020 May 22; 1-30.doi: 10.1080/22221751.2020.1772678.Online ahead of print.
3. Liu Y, Ning Z, Chen Y, Guo M, Liu Y, Gali NK, Sun L, Duan Y, Cai J, Westerdahl D, Liu X, Xu K, Ho KF*, Kan H*, Fu Q*, Lan K*. Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals. Nature. 2020 Apr 27. doi: 10.1038/s41586-020-2271
4. Liu W, Zhang Q, Chen J, Xiang R, Song H, Shu S, Chen L, Liang L, Zhou J, You L, Wu P, Zhang B, Lu Y, Xia L, Huang L, Yang Y, Liu F, Semple MG, Cowling BJ, Lan K*, Sun Z*, Yu H*, Liu Y*. Detection of Covid-19 in Children in Early January 2020 in Wuhan, China. N Engl J Med. 2020 Apr 2;382(14):1370-1371. doi: 10.1056/NEJMc2003717
5. Xiong Y, Liu Y, Cao L, Wang D, Guo M, Jiang A, Guo D, Hu W, Yang J, Tang Z, Wu H, Lin Y, Zhang M, Zhang Q, Shi M, Liu Y, Zhou Y*, Lan K*, Chen Y*. Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients. Emerg Microbes Infect. 2020 Dec;9(1):761-770. doi: 10.1080/22221751.2020.1747363.
6. Chen L, Liu W, Zhang Q, Xu K, Ye G, Wu W, Sun Z, Liu F, Wu K, Zhong B, Mei Y, Zhang W, Chen Y, Li Y, Shi M, Lan K*, Liu Y*. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerg Microbes Infect. 2020 Feb 5;9(1):313-319. doi: 10.1080/22221751.2020.1725399. eCollection 2020.
7. Wei X, Bai L, Dong L, Liu H, Xing P, Zhou Z, Wu S，Lan K*，NCOA2 promotes lytic reactivation of Kaposi’s sarcoma-associated herpesvirus by enhancing the expression of the master switch protein RTA. PLoS Pathog 2019 15(11): e1008160.
8. Tang Q, Li S, Du L, Chen S, Gao J, Cai Y, Xu Z, Zhao, Z, Lan K*, Wu S*, Emetine protects mice from enterovirus infection by inhibiting viral translation, Antiviral Research 2019, 104650 doi: https://doi.org/10.1016/j.antiviral.2019.104650.
9. Bai L, Dong J, Liu Z, Rao Y, Feng P*, Lan K*. Viperin catalyzes methionine oxidation to promote protein expression and function of helicases. Sci Adv2019(5): eaax1031
10. Zhang F, Liang D, Lin X, Zou Z, Sun R,Wang X, Liang X, Kaye KM, Lan K*.NDRG1 facilitates the replication and persistence of Kaposi'ssarcoma-associated herpesvirus by interacting with the DNA polymerase clamp PCNA. PLoS Pathog. 2019 Feb27;15(2): e1007628
11. Yuan F, Gao ZQ, Majerciak V, Bai L, HuML, Lin XX, Zheng ZM*, Dong YH*, Lan K*. The crystal structure of KSHV ORF57 reveals dimeric active sites important for protein stability and function. PLoS Pathog. 2018 Aug 10;14(8): e1007232
12. Yuan F, Gao ZQ,Majerciak V, Bai L, Hu ML, Lin XX, Zheng ZM, Dong YH, Lan K*. The crystal structure of KSHV ORF57 reveals dimeric active sites important for protein stability and function. PLoS Pathog. 2018Aug 10;14(8): e1007232
13. Li G, Zhu Y, Shao D, Chang H, Zhang X, Zhou D, Gao Y, Lan K*, Deng Q*. Recombinant covalently closed circular DNA of hepatitis B virus induceslong-term viral persistence with chronic hepatitis in a mouse model. Hepatology.2018 Jan;67(1):56-70.
14. Wang X, Zou Z, Deng Z, Liang D, Zhou X, Sun R, Lan K*. Male hormones activateEphA2 to facilitate Kaposi's sarcoma-associated herpesvirus infection: Implications for gender disparity in Kaposi's sarcoma. PLoS Pathog.2017 Sep 28;13(9): e1006580.
15. Zou Z, Meng Z, Ma C, Liang D, Sun R, Lan K*. Guanylate-binding protein1 inhibits nuclear delivery of Kaposi's sarcoma-associated herpesvirus virions by disrupting formation of actin filament. J Virol. 2017 Jul 27;91(16). pii: e00632-17
16. Lin X, Sun R, Zhang F, Gao Y, Bin L, Lan K*. The Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expressionof SUMO/sentrin Specific Peptidase 6 to Facilitate Latency Establishment. JVirol. 2017 Aug 10;91(17). pii: e00806-17
17. Sun R, Tan X, Wang X, Wang X, Yang L, Robertson ES, Lan K*. Epigenetic Landscape of Kaposi's Sarcoma-Associated Herpesvirus Genome in Classic Kaposi's Sarcoma Tissues. PLoS Pathog. 2017 Jan24;13(1): e1006167
18. Li S, Hu H, He Z, Liang D, Sun R, Lan K*. Fine-Tuning of the Kaposi's Sarcoma-Associated Herpesvirus Life Cycle in Neighboring Cells through the RTA-JAG1-Notch Pathway. PLoS Pathog. 2016 Oct19;12(10): e1005900
19. Hu H, Dong J, Liang D, Gao Z, Bai L, Sun R, HuH, Zhang H, Dong Y, Lan K*. Genome-wide mapping of the binding sites and structural analysis of KSHV vIRF2 reveal it is a DNA-binding transcription factor. J Virol. 2015 Nov4;90(3):1158-68
20. Jia B, Guo M, Li G, Yu D, Zhang X, Lan K*, Deng Q*. Hepatitis B virus core protein sensitizes hepatocytes to tumor necrosis factor-induced apoptosis by suppression of the phosphorylation of mitogen-activated protein kinase kinase 7. J Virol. 2015 Feb 15;89(4):2041-51.
21. Zhao Q, Liang D, Sun R, Jia B, Xia T, Xiao H, Lan K*. Kaposi's sarcoma-associated herpesvirus-encoded replication and transcription activatorimpairs innate immunity via ubiquitin-mediated degradation of myeloidddifferentiation factor 88. J Virol. 2015 Jan;89(1):415-27
22. Hu H, Zhang H, Gao Z, Wang D, Liu G, Xu J, Lan K*, Dong Y*. Structure of the type VI secretion phospholipase effector Tle1 provides insight into itshydrolysis and membrane targeting. ActaCrystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2175-85
23. Liang D, Hu H, Li S, Dong J, Wang X, Wang Y, He L, He Z, Gao Y, Gao S, Lan K*. Oncogenic Herpesvirus KSHV Hijacks BMP-Smad1-Id Signaling to PromoteTumorigenesis. PLoS Pathog. 2014 Jul 10;10(7): e1004253.
24. Qi Z, Li G, Hu H, Yang C, Zhang X, Leng Q, Xie Y, Yu D, Zhang X, Gao Y, Lan K*, Deng Q*. Recombinant Covalently Closed Circular Hepatitis B Virus DNA Induces Prolonged Viral Persistence in Immunocompetent Mice. J Virol.2014 Jul;88(14):8045-56
25. Sun R, Liang D, Gao Y, Lan K*. Kaposi's Sarcoma-Associated Herpesvirus-Encoded LANA Interacts with Host KAP1 To Facilitate Establishment of Viral Latency. J Virol. 2014 Jul1;88(13):7331-7344.
26. Han J, Wang L, Liu J, Jin M, Hao F, Zhang P, Zhang Z, Wen D, Wu X, Liu G, Ji L, Xu D, Zhou D, Leng Q, Lan K*, Zhang C*. Co-circulation of three HA and two NAsubtypes of avian influenza viruses in Huzhou, China, April 2013: implicationfor the origin of the novel H7N9 virus. J Virol. 2014Jun;88(11):6506-11
27. Wang X, HeZ, Xia T, Li X, Liang D, Lin X, Wen H, Lan K*. Latency-Associated Nuclear Antigen of Kaposi Sarcoma-Associated Herpesvirus Promotes Angiogenesis through Targeting Notch Signaling Effector Hey1. Cancer Res. 2014 Apr 1;74(7):2026-37.
28. Wang Z, ZhuK, Bai W, Jia B, Hu H, Zhou D, Zhang X, Zhang X, Xie Y, Bourgine MM, Michel ML, Lan K*, Deng Q*. Adenoviral delivery of recombinanthepatitis B virus expressing foreign antigenic epitopes for immunotherapy ofpersistent viral infection. J Virol. 2014Mar;88(5):3004-15.
29. Han J, Jin M, Zhang P, Liu J, Wang L, Wen D, Wu X, Liu G, Zou Y, Lv X, Dong X, Shao B, Gu S, Zhou D, Leng Q, Zhang C, Lan K*. Epidemiological linkbetween exposure to poultry and all influenza A(H7N9) confirmed cases in Huzhoucity, China, March to May 2013. Euro Surveill. 2013 May16;18(20). pii: 20481.
30. Han J, Niu F, Jin M, Wang L, Liu J, Zhang P, Xie B, Wu X, Wen D, Ji L, Liu G, Yang Z, Zhang C, Zhou D, Leng Q, Lan K*. Clinical presentationand sequence analyses of HA and NA antigens of the novel H7N9 viruses. Emerg Microbes Infect. 2013May;2(5):e23.
31. Xia T, Lévy L, Levillayer F, Jia B, Li G, Neuveut C, Buendia MA, Lan K*, Wei Y*. The Four and a Half LIM-only Protein 2 (FHL2) Activates Transforming Growth Factor β (TGF-β) Signaling by Regulating Ubiquitination of the E3 Ligase Arkadia. J Biol Chem. 2013 Jan 18;288(3):1785-94.
32. Yang Y, Xia T, Li N, Zhang J, Yang Y, Cong W, Deng Q, Lan K*, Zhou W*. Combined effects of p53 and MDM2 polymorphisms on susceptibility and surgical prognosis in hepatitis B virus-related hepatocellular carcinoma. Protein Cell. 2013 Jan;4(1):71-81.
33. Lin X, Li X, Liang D, Lan K*. MicroRNAs and unusual small RNAs discovered in Kaposi's sarcoma-associated herpesvirusvirions. J Virol. 2012Dec; 86(23): 12717-30.
34. Jin Y, He Z, Liang D, Zhang Q, Zhang H, Deng Q, Robertson ES, Lan K*. Carboxyl-Terminal Amino Acids 1052 to 1082 of the Latency-Associated Nuclear Antigen (LANA) Interact with RBP-Jκ and Are Responsible for LANA-Mediated RTA Repression. J Virol. 2012 May;86(9):4956-69
35. Liang D, Lin X, Lan K*. Looking at Kaposi's Sarcoma-Associated Herpesvirus-Host Interactions from a microRNA Viewpoint.Front Microbiol. 2011; 2:271.
36. Liu Y, Sun R, Lin X, Liang D, Deng Q, Lan K*. Kaposi's sarcoma-associated herpesvirus-encoded microRNA miR-K12-11 attenuates transforming growth factor beta signaling through suppression of SMAD5. J Virol. 2012 Feb; 86(3):1372-81.
37. Li X, Liang D, Lin X, Robertson ES, Lan K*. Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8expression in endothelial cells and impairs neutrophil chemotaxis by degrading nuclear p65. J Virol. 2011 Sep;85(17):8606-15.
38. Qin Y, Li Y, Liu W, Tian R, Guo Q, Li S, Li H, Zhang D, Zheng Y, Wu L, Lan K*, Wang J*. Penicilliummarneffei-stimulated dendritic cells enhance HIV-1trans-infection and promote viral infection by activating primary CD4+ T cells. PLoS One. 2011;6(11): e27609
39. Lin X, Liang D, He Z, Deng Q, Robertson ES, Lan K*. miR-K12-7-5p encoded by Kaposi's sarcoma-associated herpesvirus stabilizes the latent state by targeting viral ORF50/RTA. PLoS One. 2011 Jan 20;6(1):e16224.
40. Liang D, Gao Y, Lin X, He Z, Zhao Q, Deng Q, Lan K*. A human herpesvirusmiRNA attenuates interferon signaling and contributes to maintenance of viral latency by targeting IKKε. Cell Res.2011 May;21(5):793-806.
41. Wang X, He B, Zhang Z, Liu T, Wang H, Li X, Zhang Q, Lan K, Lu X, Wen H*. Human herpesvirus-8 in northwestern China: epidemiology and characterization among blood donors. Virol J. 2010 Mar 17; 7:62.
42. He Z, Liu Y, Liang D, Wang Z, Robertson ES, Lan K*. Cellular corepressor TLE2inhibits replication-and-transcription- activator-mediated transactivation and lytic reactivation of Kaposi's sarcoma-associated herpesvirus. J Virol.2010 Feb;84(4):2047-62.
43. Lan K, Murakami M, Bajaj B, Kaul R, He Z, Gan R, Feldman M, Robertson ES. Inhibition of KSHV-infected primary effusion lymphomas in NOD/SCID mice by gamma-secretase inhibitor. Cancer Biol Ther.2009 Nov;8(22):2136-43.
44. Liu Y, Cao Y, Liang D, Gao Y, Xia T, Robertson ES, Lan K*. Kaposi's sarcoma-associated herpesvirus RTA activates the processivity factorORF59 through interaction with RBP-Jkappa and a cis-acting RTA responsiveelement. Virology. 2008 Oct 25;380(2):264-75.
45. Lan K, Verma SC, Murakami M, Bajaj B, Kaul R, Robertson ES. Kaposi's sarcoma herpesvirus-encoded latency-associated nuclearantigen stabilizes intracellular activated Notch by targeting the Sel10protein. Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16287-92.
46. Lan K, Murakami M, Choudhuri T, Tsai DE, Schuster SJ, Wasik MA, and Robertson E. Detection of Epstein-Barr Virus in T-CellProlymphocytic Leukemia cells in vitro. J Clin Virol. 2008;43:260-5.
47. Verma S, Lan K, Choudhuri T, Cotter MA, and Robertson E. An autonomous replicating elementwithin the KSHV genome. Cell Host Microbe. 2007Aug 16;2(2):106-18.
48. Lan K, Choudhuri T, Murakami M, Kuppers DA, and Robertson E. Intracellular Activated Notch1 Is Critical for Proliferation of KSHV Associated B Lymphoma Cell Lines in vitro. J Virol. 2006, 80(13):6411-6419.
49. Lan K, Murakami M, Choudhuri T, Kuppers D, and Robertson E. Intracellular Activated Notch1 Can Reactivate KSHV from Latency.Virology.2006, 351:393-403.
50. Lan K, Kuppers D, Verma S, Sharma N, Murakami M,and Robertson E. Induction of KSHV Latency Associated Nuclear Antigen by the Lytic Transactivator RTA: A Novel Mechanism for Establishment of Latency. J Virol. 2005;79(12):7453-65.
51. Lan K, Kuppers D, and Robertson E. KSHVReactivation is Regulated by Interaction of LANA with RBP-Jk, the MajorDownstream Effector of the Notch Signaling Pathway. J Virol. 2005;79(6):3468-78.
52. Lan K, Kuppers DA, Verma SC, and Robertson E.Kaposi’s Sarcoma-Associated Herpesvirus-Encoded Latency-Associated NuclearAntigen Inhibits Lytic Replication by Targeting Rta: a Potential Mechanism for Virus-Mediated Control of Latency. J Virol. 2004; 78:6585-6594.
